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Department Profile : |
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- Teaching biotechnology for all the undergraduate degree programme’s in the university since 1994
- Teaching biotechnology course for postgraduate students of different faculties since 1996.
- Conducting practical training programme to teach techniques in biotechnology for
faculty members of different disciplines in the university.
- Offering master degree programme in Agriculture Biotechnology since 1996 (10-15 students/year).
- Offers Ph.D in Plant Biotechnology Since 2003 (4 students/year).
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Department Activities : |
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TEACHING:
- Teaching courses for UG ( Biotech 201).
Offering degree programme in MSc(Agri) Plant Biotechnology and Ph.D in plant Biotechnology
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RESEARCH : |
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(A) Development of regeneration protocols for somatic embryogenesis for crop plants. |
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We have been able to develop protocols for somatic embryogenesis successfully for the following crops:
- Indica Rice (var. Tarar Basmati, PTB-33 and Rasi)
- Musk melon (Eden Gem and Arkajeet)
- Peanut (var. TMV-2 and JL-24)
- Tobacco (var.FCV)
- Sweet Potato (Selection-1)
- Brinjal (Pusa purple long)
- Geranium (scarlet)
- Africal violet
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Attempts were also made for developing regeneration protocols for Dolichos
lab lab, the grain legume [which is cultivated extensively in Karnataka,
and has serious problem of pod borer (Helicoverpa armigera)] to develop transgenic insect resistant crop. |
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(B) Transgenic crops: |
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We have successfully developed following
transgenic crops following Agrobacterium mediated transformation techniques.
- Tomato variety Arka vikas developed by IIHR was transformed with Cry I Ab
gene for resistant to fruit borer (Helicoverpa armigera).
- Geranium var. Scarlet is transformed with Glucanase-Chitinase gene for resistant to fungal diseases.
- Tobacco var. FCV special is transformed with Glucanase-Chitinase gene resistant
to damping off disease caused by Pythium apanidermatum and powdery mildew disease caused by Erysiphe cinchoracearum.
- 4. Peanut var.JL-24 is transformed with Glucanase-Chitinase gene for resistance to Tikka disease caused by Cercospora.
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(C). Production of Rabies vaccine in Plants: by Dr.P.H.Ramanjini Gowda |
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The following plants are transformed with Rabies vaccine gene:
- Transgenic tobacco plants with rabies vaccine genes have been developed with Agrobacterium mediated transformation.
The protein extracted (Total) from the leaves injected into mice have shown immunogenecity. The experiment carried out in
NIMHANS has clearly showed all the transgenic tobacco plants had immunogenic Rabies glycoprotein. The
antibody produced against rabies virus was found to be highly satisfactory.Further trials with higher animals will be taken up shortly.
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- Transgenic muskmelon with Rabies glycoprotein has been developed. They are being tested for expression levels in
leaves at present. The same will be tested in fruits in due course of time
to use it as edible vaccine. The experiment is actively in progress.We have obtained complete patent for this technology.
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(D). Isolation and molecular characterization of Bacillus thuringenesis: by Dr.T.K.Siddarame Gowda |
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With an aim to isolate most effective and novel Bt genes for insect
control, several Bt strains distributed in different Agroclimatic zones of Karnataka, Andhra Pradesh and Kerala have been isolated.
These strains are identified using PCR, Insect bioassay and protein profile studies.They are being studied further to isolate the genes and to use for developing transgenic crops. |
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(E). Isolation and molecular characterization of phosphate solubilizing bacteria to develop efficient biofertilizers. |
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Phosphate solubilizing bacteria from different Agro climatic conditions of
Karnataka have been isolated from Rhizosphere and non-Rhizosphere of crop plants, weeds and medicinal plants.
Screening and isolation of five efficient Tri-calcium phosphate-solubilizing bacteria has been done successfully. |
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Genomic library of one of the most efficient bacteria has been prepared.
The clone capable of regulating phosphate solubilization has been identified. The genes involved in phosphate solubilization
are being studied.
Attempts are on to identify the genes involved in phosphate solubilization in Bacillus megatherium. |
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(F). Successfully developed toxin based screening method for identifying the tolerant sunflower lines resistant to the infection of Alternaria helianthi. |
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(G). C-DNA library for finger millet leaf base has been developed: By Dr. Theerthaprasad. |
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(H). Genetic analysis of mulberry accessions using RAPD markers was developed: By Dr. Anitha Peter. |
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(I). Molecular markers in crops and Biofuel-Ethenol: By Dr.K.M.Harinikumar. |
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COLLABORATION WITH OTHER INSTITUTES: |
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- Indian Institute of Horticultural Research, Hesaraghatta, Bangalore.
- National Centre for Biological Sciences, TIFR, Bangalore.
- Tuskegee University, USA.
- Purdue University USA.
- NIMHANS, Bangalore.
- Seri-Biotech, Bangalore.
- Indo-American Hybrid Seeds, Bangalore.
- Indian Institute of Science, Bangalore.
- Veterinary Microbiology, UAS, Bangalore.
- Arizona Biodesign Institute, USA.
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